2X2E
Dynamin GTPase dimer, long axis form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-19 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.610, 81.020, 181.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.23000 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 2.630 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARPD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.200 |
Number of reflections | 44405 | |
<I/σ(I)> | 21.4 | 11.7 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 7.16 | 7.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | HANGING DROP VAPOR DIFFUSION IN 0.1M TRIS PH 8.5, 25% PEG 3350, 200MM NACL, 25-35% NAF USING A DROP SIZE OF 2-10 MICROL AND A RESERVOIR VOLUME OF 750 MICROL. CRYSTALS GREW IN 4-5 DAYS AT EITHER 17C OR 20C. |