2X26
Crystal structure of the periplasmic aliphatic sulphonate binding protein SsuA from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.891, 96.026, 142.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.620 - 1.750 |
| R-factor | 0.18784 |
| Rwork | 0.186 |
| R-free | 0.22538 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e4r |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.619 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0106) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.200 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.410 |
| Number of reflections | 57014 | |
| <I/σ(I)> | 9.7 | 1.8 |
| Completeness [%] | 99.1 | 99.2 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 22% PEG3350, 0.2M SODIUM FORMATE AT 293K IN 2 DAYS, pH 7.5 |
