2X1M

Crystal structure of Mycobacterium smegmatis methionyl-tRNA synthetase in complex with methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-23
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	209.990, 209.990, 73.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.212
Rwork	0.210
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a8h
RMSD bond length	0.005
RMSD bond angle	0.811
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.090	0.360
Number of reflections	15164
<I/σ(I)>	17.8	5.1
Completeness [%]	98.7	99.4
Redundancy	7.7	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.2 M NAH2PO4, 800 MM K2HPO4, 200 MM LI2SO4, 100 MM CAPS, PH 7.0, VAPOR DIFFUSION, HANGING DROPS, TEMPERATURE 293 K