2X1J
H71A mutant of the antibiotic resistance protein NimA from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.254, 38.962, 60.080 |
| Unit cell angles | 90.00, 113.99, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.19886 |
| Rwork | 0.196 |
| R-free | 0.25859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w3o |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.479 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.140 | 0.140 |
| Number of reflections | 14356 | |
| <I/σ(I)> | 0 | 1.7 |
| Completeness [%] | 86.6 | 90 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 10MG/ML PROTEIN, 0.6M SODIUM ACETATE, 0.1M BUFFER PH 6 (CITRATE/MES). |
