2X1G

Crystal structure of Importin13 - Mago-Y14 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978, 0.979
Spacegroup name	P 1
Unit cell lengths	82.569, 100.792, 93.923
Unit cell angles	89.83, 110.18, 90.63

Refinement procedure

Resolution	50.000 - 3.350
R-factor	0.267
Rwork	0.267
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hl6
RMSD bond length	0.007
RMSD bond angle	1.250
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.530
High resolution limit [Å]	3.350	3.350
Rmerge	0.100	0.560
Number of reflections	39374
<I/σ(I)>	5.7	1.5
Completeness [%]	96.3	96.1
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291