2X13
The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with ADP and 3phosphoglycerate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-26 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.940, 91.440, 108.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.360 - 1.740 |
R-factor | 0.18695 |
Rwork | 0.185 |
R-free | 0.21810 |
Structure solution method | MIR |
Starting model (for MR) | 2wzb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.411 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.830 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.080 | 0.260 |
Number of reflections | 40002 | |
<I/σ(I)> | 9.1 | 3 |
Completeness [%] | 98.4 | 97 |
Redundancy | 3.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1M BIS/TRIS PH 6.5 21% P2000MME |