2X12
pH-induced modulation of Streptococcus parasanguinis adhesion by Fap1 fimbriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.668, 108.668, 126.252 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.405 - 2.900 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.25050 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.816 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.040 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.130 | 0.470 |
| Number of reflections | 19567 | |
| <I/σ(I)> | 15.19 | 1.61 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.58 | 7.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 0.1 M HEPES PH 8.0, 11% (W/V) PEG 6K, 10 MM SPERMINE-TETRA HCL HANGING DROP VAPOUR DIFFUSION |
