2X0F
Structure of WsaF in complex with dTDP-beta-L-Rha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU-MSC SATURN 944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.940, 75.650, 77.480 |
| Unit cell angles | 90.00, 102.78, 90.00 |
Refinement procedure
| Resolution | 28.430 - 2.550 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x0d |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.356 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_216)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.080 | 0.410 |
| Number of reflections | 52047 | |
| <I/σ(I)> | 12.6 | 2.5 |
| Completeness [%] | 94.8 | 66.9 |
| Redundancy | 1.9 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 MM MG FORMATE, 20% PEG3350 |
