2X0E
Complex structure of WsaF with dTDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU-MSC SATURN 944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.820, 75.560, 77.710 |
| Unit cell angles | 90.00, 103.05, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.810 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x0d |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.809 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.910 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 38750 | |
| <I/σ(I)> | 13.6 | 2.55 |
| Completeness [%] | 95.1 | 68.3 |
| Redundancy | 1.9 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 0.2 MM MG FORMATE, 20% PEG3350, 293K |
