2X0C
Crystal Structure of the R7R8 domains of Talin
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-24 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.000, 66.810, 185.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.21419 |
Rwork | 0.212 |
R-free | 0.26367 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.029 |
RMSD bond angle | 2.171 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.050 | 0.400 |
Number of reflections | 33121 | |
<I/σ(I)> | 15.2 | 2.3 |
Completeness [%] | 99.7 | 98.4 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 18% PEG 8K, 100MM TRIS, 180MM SODIUM PHOSPHATE DIBASIC, PH 8.5 |