2X0A
MPD-Lysozyme structure at 55.5 keV using a TRIXXEL CsI-aSi based digital imager and the new ESRF U22 undulator source at ID15
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID15B |
Synchrotron site | ESRF |
Beamline | ID15B |
Temperature [K] | 100 |
Detector technology | FLAT PANEL |
Collection date | 2008-07-19 |
Detector | TRIXXEL |
Wavelength(s) | 0.22 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.207, 77.207, 37.767 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.300 - 1.520 |
R-factor | 0.14874 |
Rwork | 0.146 |
R-free | 0.19902 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cgi |
RMSD bond length | 0.012 |
RMSD bond angle | 1.336 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.540 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.060 | 0.240 |
Number of reflections | 17977 | |
<I/σ(I)> | 19.6 | 3.4 |
Completeness [%] | 99.2 | 89.1 |
Redundancy | 6.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | HANGING DROP METHOD AT 295K. DROP IS 1 MICROL OF 50 MG/ML PROTEIN AND 1 MICROL OF WELL SOLUTION. WELL VOLUME IS 1ML OF 70 % MPD FROM HAMPTON RESEARCH. |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | HANGING DROP METHOD AT 295K. DROP IS 1 MICROL OF 50 MG/ML PROTEIN AND 1 MICROL OF WELL SOLUTION. WELL VOLUME IS 1ML OF 70 % MPD FROM HAMPTON RESEARCH. |