Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-07 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 132.241, 132.241, 132.241 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.570 - 1.900 |
| R-factor | 0.1485 |
| Rwork | 0.147 |
| R-free | 0.17520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y0y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.100 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.380 |
| Number of reflections | 433624 | |
| <I/σ(I)> | 18 | 7 |
| Completeness [%] | 99.4 | 97.7 |
| Redundancy | 14.5 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.17 M AMMONIUM SULPHATE, 0.085 M TRIS HCL PH 8.5, 21.25% (W/V) PEG 3300, 2.0 MICROL DROPLETS TO WHICH 0.5 MICROL OF 30% GAMMA-BUTYROLACTONE IS ADDED |
