2WZM
Crystal structure of a mycobacterium aldo-keto reductase in its apo and liganded form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 65.456, 65.456, 96.844 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.700 - 1.640 |
| R-factor | 0.18029 |
| Rwork | 0.178 |
| R-free | 0.22134 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.405 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.800 | 1.710 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.060 | 0.480 |
| Number of reflections | 57130 | |
| <I/σ(I)> | 7.3 | 1.5 |
| Completeness [%] | 97.7 | 85.1 |
| Redundancy | 5.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 0.1M TRIS-HCL PH 7.6, 1.85 (NH4)2HPO4 |
