2WZK
Structure of the Cul5 N-terminal domain at 2.05A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-24 |
Detector | ADSC CCD |
Wavelength(s) | 0.97960, 0.98000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.180, 64.500, 197.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.690 - 2.050 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.22350 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 0.940 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.690 | 2.160 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.050 | 0.700 |
Number of reflections | 25439 | |
<I/σ(I)> | 7.4 | 2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% JEFFAMINE ED 2001 PH 7.0, 0.1M HEPES PH 6.8 |