2WZK
Structure of the Cul5 N-terminal domain at 2.05A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-24 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.97960, 0.98000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.180, 64.500, 197.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.690 - 2.050 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.22350 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.940 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.690 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.050 | 0.700 |
| Number of reflections | 25439 | |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% JEFFAMINE ED 2001 PH 7.0, 0.1M HEPES PH 6.8 |
