2WZJ

Catalytic and UBA domain of kinase MARK2/(Par-1) K82R, T208E double mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-28
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	162.240, 205.578, 91.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.394 - 2.786
R-factor	0.1819
Rwork	0.179
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zmu
RMSD bond length	0.008
RMSD bond angle	1.138
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.780	2.780
Rmerge	0.070	0.690
Number of reflections	76705
<I/σ(I)>	19.64	2.49
Completeness [%]	99.4	97.7
Redundancy	4.97	4.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	60% TACSIMATE PH 7, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K