2WZF
Legionella pneumophila glucosyltransferase crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 73 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.999, 104.641, 53.261 |
| Unit cell angles | 90.00, 101.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.18901 |
| Rwork | 0.188 |
| R-free | 0.25431 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wzg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.219 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 | 0.400 |
| Number of reflections | 31842 | |
| <I/σ(I)> | 23.2 | 5.5 |
| Completeness [%] | 97.1 | 91.8 |
| Redundancy | 6.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3000, 0.2 M NACL, 0.1 M HEPES, PH 7.5 |
