2WYQ
THE CRYSTAL STRUCTURE OF THE UBIQUITIN-LIKE (UBL) DOMAIN OF HHR23A (HUMAN HOMOLOGUE A OF RAD23)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 78.434, 78.434, 63.472 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 1.651 |
| R-factor | 0.126 |
| Rwork | 0.124 |
| R-free | 0.15980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bwf |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.288 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.570 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 14363 | |
| <I/σ(I)> | 5.86 | 2.08 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 36.17 | 23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 2% PEG 400 AND 2.0 M AMMONIUM SULFATE, AT ROOM TEMPERATURE |
