2WYP
Crystal structure of sialic acid binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-27 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.836, 74.489, 88.151 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.450 - 1.500 |
R-factor | 0.139 |
Rwork | 0.136 |
R-free | 0.19500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v4c |
RMSD bond length | 0.017 |
RMSD bond angle | 1.629 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.520 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.110 | 0.400 |
Number of reflections | 46426 | |
<I/σ(I)> | 11.8 | 3.8 |
Completeness [%] | 90.1 | 99.2 |
Redundancy | 6.2 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 277 | 30 MG/ML SIAP (IN 20 MM HEPES, 10 MM NACL AND KDN, PH 8.0) AND EQUAL VOLUME OF RESERVOIR SOLUTION (100MM MES PH 6.0, 28.5% PEG 6K, 200MM SODIUM THIOCYANATE), TEMPERATURE 277K |