2WYM
Structure of a metallo-b-lactamase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2008-07-27 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.221, 178.212, 111.559 |
Unit cell angles | 90.00, 103.96, 90.00 |
Refinement procedure
Resolution | 14.970 - 2.600 |
R-factor | 0.21 |
Rwork | 0.207 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wyl |
RMSD bond length | 0.006 |
RMSD bond angle | 0.868 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.500 | 2.670 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.110 | |
Number of reflections | 60423 | |
<I/σ(I)> | 10.4 | 3.19 |
Completeness [%] | 91.5 | 70.5 |
Redundancy | 8.3 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% PEG 3350, 0.2M LITHIUM CITRATE |