2WYK
SiaP in complex with Neu5Gc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-20 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.407, 74.800, 87.334 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.040 - 1.500 |
R-factor | 0.13488 |
Rwork | 0.132 |
R-free | 0.17910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v4c |
RMSD bond length | 0.017 |
RMSD bond angle | 1.601 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.110 | 0.560 |
Number of reflections | 49622 | |
<I/σ(I)> | 19.4 | 2.3 |
Completeness [%] | 98.4 | 96.1 |
Redundancy | 8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 277 | 30 MG/ML SIAP (IN 20 MM HEPES, 10 MM NACL, PH 8.0) AND EQUAL VOLUME OF RESERVOIR SOLUTION (100MM MES PH 6.0, 28.5% PEG 6K, 3% W/V 1,5-DIAMINOPENTANE DIHYDROCHLORIDE), TEMPERATURE 277K |