2WY1
Crystal structure of rat angiotensinogen in P321 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 133.103, 133.103, 97.185 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 115.470 - 3.150 |
| R-factor | 0.2776 |
| Rwork | 0.275 |
| R-free | 0.32728 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.903 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0099) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.500 | 3.320 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.160 | 0.990 |
| Number of reflections | 17386 | |
| <I/σ(I)> | 11.9 | 1.1 |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 7.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 15-20% PEG4000, 0.2M MGCL2, 0.1M TRIS, PH8.5 |
