2WXW
Crystal structure of human angiotensinogen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 133.285, 133.285, 123.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.070 - 3.300 |
| R-factor | 0.31 |
| Rwork | 0.309 |
| R-free | 0.31800 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.759 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0099) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.500 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.060 | 0.790 |
| Number of reflections | 17197 | |
| <I/σ(I)> | 9.5 | 4.5 |
| Completeness [%] | 97.5 | 89.9 |
| Redundancy | 9.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 2-2.5N NACL, 0.2M CACL2, 0.1M TRIS-HCL, PH8 |
