2WXT
Clostridium perfringens alpha-toxin strain NCTC8237
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-11-25 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.180, 174.370, 79.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.062 - 2.000 |
| R-factor | 0.1778 |
| Rwork | 0.175 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ca1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.346 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.350 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.230 |
| Number of reflections | 25562 | |
| <I/σ(I)> | 11.3 | 4 |
| Completeness [%] | 89.3 | 91.6 |
| Redundancy | 4.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 1 M NA ACETATE, 0.1 M NA-HEPES, PH7.6, 0.05 M CDSO4 |
