2WX9
Crystal structure of the sialic acid binding periplasmic protein SiaP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-13 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.730, 74.490, 87.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.250 - 1.370 |
R-factor | 0.118 |
Rwork | 0.116 |
R-free | 0.15800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v4c |
RMSD bond length | 0.017 |
RMSD bond angle | 1.681 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.250 | 1.450 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.080 | 0.620 |
Number of reflections | 62379 | |
<I/σ(I)> | 12.8 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 277 | 36 MG/ML SIAP (IN 20 MM HEPES, 10 MM NACL,AND NEU5GC PH 8.0) AND EQUAL VOLUME OF RESERVOIR SOLUTION (100MM MES PH 6.0, 28.5% PEG 6K, 3% W/V 1,5-DIAMINOPENTANE DIHYDROCHLORIDE) TEMPERATURE 277K |