2WWX

Crystal structure of the SidM/DrrA(GEF/GDF domain)-Rab1(GTPase domain) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	93
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	68.880, 73.803, 73.665
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.500
R-factor	0.21265
Rwork	0.212
R-free	0.23100
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	1.134
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.060	0.120
Number of reflections	56689
<I/σ(I)>	59.7	13.9
Completeness [%]	98.3	96.7
Redundancy	10.6	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1