2WWM
Crystal structure of the Titin M10-Obscurin like 1 Ig complex in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.650, 44.940, 68.010 |
| Unit cell angles | 87.29, 86.91, 68.79 |
Refinement procedure
| Resolution | 35.880 - 2.300 |
| R-factor | 0.19997 |
| Rwork | 0.197 |
| R-free | 0.25219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wp3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.235 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.130 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.250 |
| Number of reflections | 17094 | |
| <I/σ(I)> | 13.2 | 4.3 |
| Completeness [%] | 92.6 | 93.1 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M MIB BUFFER PH 5.0, 25% PEG 1500 |
