2WVU
Crystal structure of a Michaelis complex of alpha-L-fucosidase GH29 from Bacteroides thetaiotaomicron with the synthetic substrate 4- nitrophenyl-alpha-L-fucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2009-05-11 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.610, 184.820, 98.060 |
Unit cell angles | 90.00, 94.63, 90.00 |
Refinement procedure
Resolution | 47.250 - 1.950 |
R-factor | 0.18115 |
Rwork | 0.179 |
R-free | 0.22889 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.344 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.950 |
Rmerge | 0.100 |
Number of reflections | 138108 |
<I/σ(I)> | 9.2 |
Completeness [%] | 98.4 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |