2WVO
Structure of the HET-S N-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.260, 95.260, 170.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.100 - 2.300 |
R-factor | 0.22705 |
Rwork | 0.225 |
R-free | 0.25693 |
Structure solution method | MIR |
Starting model (for MR) | 2wvn |
RMSD bond length | 0.010 |
RMSD bond angle | 1.411 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.050 | 0.570 |
Number of reflections | 40556 | |
<I/σ(I)> | 23.8 | 3.6 |
Completeness [%] | 99.3 | 99.1 |
Redundancy | 7 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 292 | CRYSTALLIZED AT 292K IN 50 MM HEPES PH 7.5, 4.0 M NACL |