2WV3
Neuroplastin-55 binds to and signals through the fibroblast growth factor receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 49.390, 81.080, 137.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.601 - 1.950 |
| R-factor | 0.2097 |
| Rwork | 0.208 |
| R-free | 0.24860 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.600 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.370 |
| Number of reflections | 20597 | |
| <I/σ(I)> | 8.3 | 2.1 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | NEUROPLASTIN-55 AT A CONCENTRATION OF 4 MG/ML IN 5 MM SODIUM PHOSPHATE AND 150 MM NACL, PH 7.4, WAS MIXED WITH THE RESERVOIR SOLUTION CONTAINING 13-15% W/V PEG 4000, 0.2 M AMMONIUM SULFATE, AND 0.1 M SODIUM ACETATE, PH 4.6. |
