2WU5
Crystal structure of the E. coli succinate:quinone oxidoreductase (SQR) SdhD His71Met mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 120.060, 183.820, 203.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.827 - 2.803 |
| R-factor | 0.2153 |
| Rwork | 0.213 |
| R-free | 0.25150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wdq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.565 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.970 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.570 |
| Number of reflections | 110749 | |
| <I/σ(I)> | 12.2 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS PH 8.5, 0.1M MGSO4, 11.5% PEG4000, 1MM CARBOXIN |
