2WU0

Crystal Structure Analysis of Klebsiella sp ASR1 Phytase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	81.713, 122.929, 205.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.150 - 2.570
R-factor	0.20074
Rwork	0.198
R-free	0.24301
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.218
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.150
High resolution limit [Å]	2.570
Rmerge	0.080
Number of reflections	66228
Completeness [%]	99.2
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				12% PEG8000, 0.08 M (NH4)2SO4, 0.1 M SODIUM ACETATE, 1.5 MM PHYTATE