2WTU
Crystal structure of Escherichia coli MutS in complex with a 16 basepair oligo containing an A.A mismatch.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.105, 137.890, 161.446 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.400 |
| R-factor | 0.20625 |
| Rwork | 0.203 |
| R-free | 0.26303 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oh6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.111 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.150 | 0.470 |
| Number of reflections | 28641 | |
| <I/σ(I)> | 13.6 | 4.3 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 18% PEG 3350, 100 MM SODIUM CITRATE, 100 MM BIS TRIS PROPANE PH 7.5, 5 MM MGCL2, 100 MICROM ADP. |
