2WTP
Crystal Structure of Cu-form Czce from C. metallidurans CH34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.000, 29.530, 71.170 |
| Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
| Resolution | 19.141 - 1.500 |
| R-factor | 0.1613 |
| Rwork | 0.160 |
| R-free | 0.18850 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.448 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.140 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.240 |
| Number of reflections | 32477 | |
| <I/σ(I)> | 17.6 | 5.5 |
| Completeness [%] | 99.4 | 97.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | PEG4K, MGCL2, TRIS PH 8.5, GLYCEROL |
