2WTO
Crystal Structure of Apo-form Czce from C. metallidurans CH34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.430, 29.610, 70.960 |
| Unit cell angles | 90.00, 113.75, 90.00 |
Refinement procedure
| Resolution | 48.249 - 1.850 |
| R-factor | 0.1649 |
| Rwork | 0.164 |
| R-free | 0.19210 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.325 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 1.940 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.040 | 0.130 |
| Number of reflections | 17623 | |
| <I/σ(I)> | 22.8 | 3.6 |
| Completeness [%] | 99.6 | 97.3 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | PEG4K, MGCL2, TRIS PH 8.5, GLYCEROL |
