2WTM
Est1E from Butyrivibrio proteoclasticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.035, 109.067, 91.647 |
Unit cell angles | 90.00, 102.18, 90.00 |
Refinement procedure
Resolution | 89.440 - 1.600 |
R-factor | 0.15793 |
Rwork | 0.156 |
R-free | 0.19410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 1.905 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.650 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.080 | 0.350 |
Number of reflections | 124101 | |
<I/σ(I)> | 13.7 | 4.8 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |