2WTM
Est1E from Butyrivibrio proteoclasticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.035, 109.067, 91.647 |
| Unit cell angles | 90.00, 102.18, 90.00 |
Refinement procedure
| Resolution | 89.440 - 1.600 |
| R-factor | 0.15793 |
| Rwork | 0.156 |
| R-free | 0.19410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.905 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 124101 | |
| <I/σ(I)> | 13.7 | 4.8 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
