2WTG
High resolution 3D structure of C.elegans globin-like protein GLB-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.947, 81.947, 47.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.640 - 1.500 |
| R-factor | 0.16699 |
| Rwork | 0.163 |
| R-free | 0.20118 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.431 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.600 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.100 |
| Number of reflections | 25735 |
| <I/σ(I)> | 30.7 |
| Completeness [%] | 98.1 |
| Redundancy | 28.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | PEG 4K 10%, 0.1 M SODIUM ACETATE PH 5.5 |
