2WTB
Arabidopsis thaliana multifuctional protein, MFP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2007-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 110.480, 110.480, 125.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.279 - 2.500 |
| R-factor | 0.2183 |
| Rwork | 0.216 |
| R-free | 0.26880 |
| Structure solution method | SAD |
| Starting model (for MR) | 1wdk |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.581 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.300 | 2.670 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.071 | 0.360 |
| Number of reflections | 34691 | |
| <I/σ(I)> | 6 | 2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 4.2M NACHO2, 2(V/V)% GLYCEROL, 40(V/V)% POLYPROPYLENE GLYCOL, pH 7.5 |
