2WT8
Structure of the N-terminal BRCT domain of human microcephalin (Mcph1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.955, 34.215, 84.230 |
| Unit cell angles | 90.00, 113.53, 90.00 |
Refinement procedure
| Resolution | 45.248 - 1.600 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.22210 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.057 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.270 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.340 |
| Number of reflections | 56453 | |
| <I/σ(I)> | 16.1 | 3.2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM MES, PH 6.0, 18% PEG 6000, 200 MM NH4CL |
