2WT1
Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre complexed with lacto-N-neo-tetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-26 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.420, 38.790, 84.400 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.1894 |
| Rwork | 0.188 |
| R-free | 0.22296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2WSU CHAIN A |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.369 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 23751 | |
| <I/σ(I)> | 12.6 | 3.2 |
| Completeness [%] | 98.5 | 91.7 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10 MM TRIS-HCL PH 8.0, 27% (W/V) POLY -ETHYLENE GLYCOL 3350, 200 MM LITHIUM NITRATE 5 MM DITHIOTHREITOL, 5 MM LACTO-N-NEO-TETRAOSE |
