2WT0
Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre complexed with N-acetyl-lactosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-17 |
| Detector | MAR555 FLAT PANEL |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.450, 43.650, 63.900 |
| Unit cell angles | 90.00, 105.82, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.910 |
| R-factor | 0.18443 |
| Rwork | 0.182 |
| R-free | 0.23799 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wsu CHAIN A |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.345 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.010 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.100 | 0.310 |
| Number of reflections | 22025 | |
| <I/σ(I)> | 5.9 | 2.7 |
| Completeness [%] | 98.0 | 98.5 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 35% (W/V) PEG 3350, 500 MM SODIUM NITRATE, 5 MM DITHIOTHREITOL, 40 MM N-ACETYL-LACTOSAMINE, 10 MM TRIS-HCL 8.0 |
