2WST
Head domain of porcine adenovirus type 4 NADC-1 isolate fibre
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2008-07-02 |
| Detector | ENRAF-NONIUS KAPPACCD 2000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 125.729, 145.439, 147.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.210 - 3.200 |
| R-factor | 0.17442 |
| Rwork | 0.173 |
| R-free | 0.20138 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1knb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.320 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.070 | 0.310 |
| Number of reflections | 45035 | |
| <I/σ(I)> | 17.5 | 2.9 |
| Completeness [%] | 98.9 | 97.5 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10 MM TRIS-HCL PH 8.0, 1 MM EDTA, 1-10% (W/V) POLY-ETHYLENE GLYCOL 8000, 1 M LITHIUM SULPHATE |
