2WSR
MONOTIM MUTANT RMM0-1, MONOMERIC FORM.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-16 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.617, 41.976, 69.512 |
| Unit cell angles | 90.00, 108.69, 90.00 |
Refinement procedure
| Resolution | 20.240 - 1.650 |
| R-factor | 0.2155 |
| Rwork | 0.211 |
| R-free | 0.25548 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tri |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.780 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.940 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.090 | 0.390 |
| Number of reflections | 32843 | |
| <I/σ(I)> | 4.1 | 1.5 |
| Completeness [%] | 91.0 | 88.4 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 291 | 100 MM MES BUFFER PH 6.2, 180 MM LI2SO4, 26 PERCENT PEG 6000, 5 MM DITHIOTHREITOL, 1 MM EDTA AND 1 MM NAN3, AT 18 DEGREES CELSIUS. |
