2WSP
Thermotoga maritima alpha-L-fucosynthase, TmD224G, in complex with alpha-L-Fuc-(1-2)-beta-L-Fuc-N3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-04 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 180.249, 180.249, 169.733 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 114.900 - 2.650 |
| R-factor | 0.20338 |
| Rwork | 0.201 |
| R-free | 0.24059 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hl8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.949 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 30789 | |
| <I/σ(I)> | 16.5 | 3.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18 % PEG600, 5 % JEFFAMINE M-600, 100 MM TRIS-HCL PH 8.0, 14 MG/ML BETA-L-FUC-N3 PROTEIN CONC. 5 MG/ML |
