2WSO
Structure of Cerulean Fluorescent Protein at physiological pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-02 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.990, 62.810, 69.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.570 - 1.150 |
R-factor | 0.14689 |
Rwork | 0.145 |
R-free | 0.17707 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oxe |
RMSD bond length | 0.018 |
RMSD bond angle | 1.881 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.220 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.040 | 0.450 |
Number of reflections | 78022 | |
<I/σ(I)> | 20.4 | 2.8 |
Completeness [%] | 97.7 | 92.1 |
Redundancy | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 15% PEG 8000, 0.1M MGCL2, 0.1 M HEPES PH 7.0 |