2WSN
Structure of Enhanced Cyan Fluorescent Protein at physiological pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.182, 62.720, 70.593 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.290 - 1.370 |
| R-factor | 0.14248 |
| Rwork | 0.140 |
| R-free | 0.18514 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oxe |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.853 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.420 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.050 | 0.410 |
| Number of reflections | 45997 | |
| <I/σ(I)> | 22.3 | 3.3 |
| Completeness [%] | 94.9 | 70.4 |
| Redundancy | 4.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 19.5% PEG8000, 4% GLYCEROL, 1M HEPES PH 7.5 |
