2WSM

Crystal structure of Hydrogenase Maturation Factor HypB From Archaeoglobus Fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	110
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 2
Unit cell lengths	72.494, 82.325, 68.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.640 - 2.300
R-factor	0.226
Rwork	0.223
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hf8
RMSD bond length	0.005
RMSD bond angle	0.829
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.760	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.110	0.250
Number of reflections	18861
<I/σ(I)>	12.5	5.4
Completeness [%]	100.0	100
Redundancy	20	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1