2WSD
Proximal mutations at the type 1 Cu site of CotA-laccase: I494A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.868, 101.868, 137.037 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.990 - 1.600 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w6l |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.158 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.064 | 0.363 |
| Number of reflections | 107969 | |
| <I/σ(I)> | 18.1 | 2.6 |
| Completeness [%] | 99.4 | 98.4 |
| Redundancy | 5.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 10% PEG MME 5K, 0.1M SODIUM CITRATE PH 5.5, 14% ISOPROPANOL |
