2WSC
Improved Model of Plant Photosystem I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-12 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 120.655, 189.086, 129.388 |
Unit cell angles | 90.00, 91.24, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.300 |
R-factor | 0.3627 |
Rwork | 0.363 |
R-free | 0.36594 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1YO9 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MR |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.430 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.100 | 0.400 |
Number of reflections | 88964 | |
<I/σ(I)> | 17.2 | 2.8 |
Completeness [%] | 99.0 | 95.2 |
Redundancy | 4.3 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 22.5 MM MES BIS-TRIS PH 6 10MM SUCCINIC ACID 6% PEG6000 0.015% DTM |