2WS6
Semi-synthetic analogue of human insulin NMeTyrB26-insulin in hexamer form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-30 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.962, 61.927, 47.297 |
Unit cell angles | 90.00, 111.90, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.500 |
R-factor | 0.15244 |
Rwork | 0.150 |
R-free | 0.19951 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mso |
RMSD bond length | 0.025 |
RMSD bond angle | 2.173 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0082) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.050 | 0.480 |
Number of reflections | 50473 | |
<I/σ(I)> | 11.7 | 5 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | 6% TRIS PH 8.2, 0.1 M NA CITRATE, 0.02% ZN ACETATE, 0.06% PHENOL |