2WS6
Semi-synthetic analogue of human insulin NMeTyrB26-insulin in hexamer form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.962, 61.927, 47.297 |
| Unit cell angles | 90.00, 111.90, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.500 |
| R-factor | 0.15244 |
| Rwork | 0.150 |
| R-free | 0.19951 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mso |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.173 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0082) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 50473 | |
| <I/σ(I)> | 11.7 | 5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 6% TRIS PH 8.2, 0.1 M NA CITRATE, 0.02% ZN ACETATE, 0.06% PHENOL |
