2WRW
Semi-synthetic highly active analogue of human insulin D-ProB26-DTI- NH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-19 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 39.372, 39.372, 123.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.410 |
| R-factor | 0.23931 |
| Rwork | 0.237 |
| R-free | 0.30086 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mso |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.632 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.060 | 0.220 |
| Number of reflections | 2438 | |
| <I/σ(I)> | 14.6 | 9.2 |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3 | 0.015 M CS2SO4 PH 3.0 |
